ber act8 3151011b cd123 il3r  (fluidigm)

Journal: EMBO Molecular Medicine

Article Title: LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis

doi: 10.1038/s44321-024-00106-1

Figure Lengend Snippet: ( A ) Correlation analysis of expression between LILRB1 and other markers including GZMB, PRF1, IFN-γ, and TNF-α based on CyTOF single-cell data ( n = 35,000 cells). ( B – D ) Percentages of CD107a + ( B ), IFN-γ + ( C ), or TNF-α + ( D ) cells within total NK cells. ( E , F ) Supernatant levels of GZMB ( E ) and PRF1 ( F ) in MDM-NK cell co-cultures. ( G ) Percentages of apoptotic cells within total NK cells. ( H ) Mtb survival in MDM-NK cell co-cultures. For ( B – H ), HC donor-derived NK cells with overexpression of MSCV or MSCV-LILRB1 were co-cultured with or without Mtb-infected MDMs (Mtb-Mφ) for 24 h. ( I ) Histological and immunofluorescence analysis of human lung sections containing non-necrotic or necrotic TB granulomas or healthy control tissues adjacent to granulomatous lesions. Left, representative images (scale bars, 200 μm) for hematoxylin and eosin (H&E) staining. Right, representative images (scale bars, 10 μm) for cells stained with antibodies against LILRB1 (blue), CD56 (green), and CD69 or CD103 (red). Nuclei were stained with DAPI (gray). ( J , K ) Quantitation of LILRB1 expression in CD56 + CD69 + or CD56 + CD69 – NK cells ( J ) and CD56 + CD103 + or CD56 + CD103 – NK cells ( K ). For ( I – K ), lung sections from 5 ATB patients were examined. Data are mean ± SEM ( n = 5 donors per group) in ( B – H , J , K ). Statistical significance was determined using two-way ANOVA ( B – G , J , K ) or one-way ANOVA ( H ) with Tukey’s post-hoc test. Results are representative of three independent experiments. .

Article Snippet: Mouse anti-human CD103 (B-Ly7) , Invitrogen , 14-1038-82.

Techniques: Expressing, Derivative Assay, Over Expression, Cell Culture, Infection, Immunofluorescence, Control, Staining, Quantitation Assay

Journal: EMBO Molecular Medicine

Article Title: LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis

doi: 10.1038/s44321-024-00106-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Mouse anti-human CD103 (B-Ly7) , Invitrogen , 14-1038-82.

Techniques: Recombinant, Western Blot, Sequencing, Modification, Cell Isolation, Enzyme-linked Immunosorbent Assay, Isolation, Antibody Labeling, Transfection, Lysis, Protease Inhibitor, Blocking Assay, Software, Flow Cytometry, Cytometry

Journal: Cell Reports Medicine

Article Title: Mitochondrial DNA-boosted dendritic cell-based nanovaccination triggers antitumor immunity in lung and pancreatic cancers

doi: 10.1016/j.xcrm.2024.101648

Figure Lengend Snippet: cNP DT6066 @MV DC treatment converts immune-cold pancreatic tumors into hot tumors, reduces hypoxia, and enhances blood vessel function (A) FACS analysis of CD11c + CD86 + DCs in orthotopic DT6066 pancreatic tumors after treatments, with a bar chart showing their percentage relative to total lymphocytes ( n = 3 mice per group). (B) FACS analysis of CD3 + CD4 + CD69 + T cells in orthotopic pancreatic tumors after treatments, with a bar chart showing their percentage relative to total CD3 + CD4 + T cells in each group ( n = 3 mice per group). (C) FACS analysis of CD3 + CD8 + CD69 + T cells in orthotopic pancreatic tumors after treatments, with a bar chart showing their percentage relative to total CD3 + CD8 + T cells in each group ( n = 3 mice per group). (D) Representative images of CD11c and CD86 co-immunostaining in tumor sections from each treatment group. Bar chart shows the quantification of CD11c + CD86 + DC × 10 3 per cm 2 in each group ( n = 3 mice per group). (E and F) Representative images of co-immunostaining for CD3 and CD4 (E) or CD8 (F) in tumor sections from each treatment group ( n = 3 mice per group). (G) FACS analysis of CD3 − NK1.1 + NK cells or CD3 − NK1.1 + CD69 + NK cells in orthotopic pancreatic tumors after treatments. Bar charts show their percentage relative to total CD3 − cells (left) or CD3 − NK1.1 + NK cells (right) in each group ( n = 3 mice per group). (H–J) Cytotoxicity LDH release assays of DT6066 cells after co-culture with various ratios of CD3 + CD8 + T cells isolated from tumors (H), lymph nodes (I), or spleens (J) of orthotopic DT6066 pancreatic tumor-bearing mice after treatments ( n = 3 mice per group). (K) FACS analysis of migratory DCs in orthotopic pancreatic tumors and TDLNs from mice treated as indicated. Bar charts show CD86 + CD103 + DCs as a percentage of CD11c + DCs in each group ( n = 3 mice per group). (L) Representative images of CD11c/CD86/CD103 triple immunostaining on paired tumor and TDLN sections in each group. Bar chart shows the percentage of CD11c + CD86 + CD103 + migratory DC × 10 3 per cm 2 in tumors (left) or TDLNs (right) from each group ( n = 3 mice per group). (M–O) Representative microbubble contrast ultrasound images are shown, and bar charts show quantification across the entire tumors, including tumor cores ( n = 3 mice per group). (P) Representative IHC staining of endomucin in tumor sections from each treatment group. Bar chart shows blood vessel diameter (left) or number of blood vessels (right) per cm 2 in each group ( n = 3 mice per group). (Q) Representative image of GLUT1 and endomucin co-immunostaining in tumor sections from each group. Bar chart shows the relative GLUT1 intensity per group ( n = 3 mice per group). (A–G, K, L, and N–Q) One-way ANOVA. (H–J) Two-way ANOVA. Scale bars in (D), (E), (F), and (L) represent 50 μm. (M) 1 cm. (P and Q) 100 μm. See also Figures S5 and .

Article Snippet: rabbit mAb against human CD103 , CST , Cat# 95835S; RRID: AB_3105887.

Techniques: Immunostaining, Co-Culture Assay, Isolation, Triple Immunostaining, Immunohistochemistry

Journal: Cell Reports Medicine

Article Title: Mitochondrial DNA-boosted dendritic cell-based nanovaccination triggers antitumor immunity in lung and pancreatic cancers

doi: 10.1016/j.xcrm.2024.101648

Figure Lengend Snippet:

Article Snippet: rabbit mAb against human CD103 , CST , Cat# 95835S; RRID: AB_3105887.

Techniques: Virus, Recombinant, Negative Staining, Lysis, Control, Phospho-proteomics, Cell Isolation, DNA Purification, DNA Extraction, Bicinchoninic Acid Protein Assay, Protein Purification, Magnetic Beads, Software

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures

doi: 10.1007/s00262-024-03749-8

Figure Lengend Snippet: Phenotype analysis of peripheral blood lymphocytes and matched tumor infiltrating lymphocytes form CRC patients. A Typical results of flow cytometry analysis revealed the frequency of CD39 + CD103 + CD8 + T cells in PBLs and matched TILs of CRC patients. B Statistical analysis of the frequency of CD39 + CD103 + T cells in PBLs and matched TILs of CRC patients. C Typical results of the frequency of CD8 + PD-1 + T cells in PBL and matched TILs of CRC patients. D Statistical analysis of the frequency of CD8 + PD-1 + T cells in PBLs and matched TILs of CRC patients. E Typical results of the frequency of CD8 + CXCL13 + T cells in PBLs and matched TILs of CRC patients. F. Statistical analysis of the frequency of CD8 + CXCL13 + T cells in PBLs and matched TILs of CRC patients

Article Snippet: For CD39 + CD103 + positive T cell analysis, TILs and PBLs from CRC patients were stained with anti-human CD3-FITC, anti-human CD8-PE, anti-human CD39-PE-Cy7, anti-human CD103-APC antibodies cocktails and 7AAD (Invitrogen).

Techniques: Flow Cytometry